Development of a scanning angle total internal reflection Raman spectrometer
نویسندگان
چکیده
منابع مشابه
Near IR scanning angle total internal reflection Raman spectroscopy at smooth gold films.
Total internal reflection (TIR) Raman and reflectivity spectra were collected for nonresonant analytes as a function of incident angle at sapphire or sapphire/smooth 50 nm gold interfaces using 785 nm excitation. For both interfaces, the Raman signal as a function of incident angle is well-modeled by the calculated interfacial mean square electric field (MSEF) relative to the incident field tim...
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Total internal reflection (TIR) Raman spectroscopy is an experimentally straightforward, surface-sensitive technique for obtaining chemically specific spectroscopic information from a region within approximately 100-200 nm of a surface. While TIR Raman spectroscopy has long been overshadowed by surface-enhanced Raman scattering, with modern instrumentation TIR Raman spectra can be acquired from...
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Conventional lens stops, implemented with an absorptive physical aperture, have an angle-dependent projection that introduces field dependent loss and reduces diffraction-limited resolution. Retro-telephoto lenses obtain uniform response using aberration vignetting, but this results in low wide-angle resolution and significant lens volume. However, an angle-independent "virtual" aperture can be...
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Total internal reflection fluorescence microscopy (TIRFM) is the method of choice to visualize a variety of cellular processes in particular events localized near the plasma membrane of live adherent cells. This imaging technique not relying on particular fluorescent probes provides a high sectioning capability. It is, however, restricted to a single plane. We present here a method based on a v...
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Two-photon fluorescence microscopy is a powerful method for many applications. Because of the ability of strong attenuation of the background fluorescence, total internal reflection fluorescence microscopy (TIRFM) has been the major technique in the fluorescence imaging and single molecule detections. Recently, we have developed a new type of TIRFM called scanning TIRFM (henceforth abbreviated ...
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ژورنال
عنوان ژورنال: Review of Scientific Instruments
سال: 2010
ISSN: 0034-6748,1089-7623
DOI: 10.1063/1.3378682